Testing Proteins with Biurets Reagent

Testing Proteins with Biurets Reagent Introduction Protein is a major and indispensable class of cellular macromolecule . There be some proteins that inspection and repair stick out cells and some others that ar enzymes which help increase the set up of cellular reactions. non only do proteins provide vital functions in alive organisms but they are also used to transpose substances surrounded by membranes and help regulate activity of other proteins and DNA. They either feed as hormones or help make up against infectious microorganisms. These proteins brood of macromolecules which are polymers consisting of one or much chains that are un-branched from monomers that are called amino acids. This molecule consists of some(prenominal) an amino and a carboxyl stem. Proteins can contain from as few as three to fifty-thousand amino acid units and rake from shape and form, to organism completely soluble or water-insoluble to water solutions. When a protein is forme d it is linked together by a peptide sequester using covalent bonds to form amidst an amino group of one amino acid and a carboxyl group. likewise dipeptides are formed when the peptide bond joins the dickens amino acids. Futhermore protiens are also linked by linking three amino acids and this is called a polypeptide.

The purpose of this experiment was to determine protein minginess in antithetic kind of substances using Biurets Reagent and assess if peptide bonds were present.If Biurets Reagent is added to the substances and so the substances pull up stakes smorgasbord to the color of Biurets Reagent. When adding Biurets Reage nt to the different substances it will endp! oint in a change of color of blue depending on how many peptide bonds exist. If none exist then there will be no change in the substance. Materials The materials that were used in this experiment were instruments such(prenominal) as: Beakers disposable pipettes, test provides, test tube holders, Chinese markers. The substances that were used were: Distilled H2O, glucose, starch, egg whites, sports drink,...If you want to get a replete(p) essay, order it on our website: BestEssayCheap.com

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